Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells |
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| Authors: | Coso Sanja Zeng Yiping Sooraj Dhanya Williams Elizabeth D |
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| Affiliation: | aCentre for Cancer Research, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia |
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| Abstract: | Lymphatic vessels guide interstitial fluid, modulate immune responses by regulating leukocyte and antigen trafficking to lymph nodes, and in a cancer setting enable tumor cells to track to regional lymph nodes. The aim of the study was to determine whether primary murine lymphatic endothelial cells (mLECs) show conserved vascular endothelial growth factor (VEGF) signaling pathways with human LECs (hLECs). LECs were successfully isolated from murine dermis and prostate. Similar to hLECs, vascular endothelial growth factor (VEGF) family ligands activated MAPK and pAkt intracellular signaling pathways in mLECs. We describe a robust protocol for isolation of mLECs which, by harnessing the power of transgenic and knockout mouse models, will be a useful tool to study how LEC phenotype contributes to alterations in lymphatic vessel formation and function. |
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| Keywords: | Abbreviations: BSA, bovine serum albumin BVEC, blood vascular endothelial cell EBM, endothelial basal media EGM, endothelial growth media ECM, extracellular matrix FBS, fetal bovine serum HUVEC, human umbilical vein endothelial cell LEC, lymphatic endothelial cell VEGF, vascular endothelial growth factor VEGFR, vascular endothelial growth factor receptor |
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