Cryptosporidium parvum: Radiation-induced alteration of the oocyst proteome |
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Authors: | Soo-Ung Lee Migyo Joung Taekyoung Nam Woo-Yoon Park Young-Hoon Ji Jae-Ran Yu |
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Institution: | aDepartment of Environmental and Tropical Medicine, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea;bDepartment of Radiation Oncology, College of Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea;cDivision of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul 215-4, Republic of Korea |
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Abstract: | Cryptosporidium parvum is a waterborne protozoan parasite that is found intracellularly in host animals, including humans, and causes severe diarrhea, which can lead to the death of an immunocompromised individual. Previously, we found that this organism is highly radioresistant as it can productively infect mice after exposure to a 10-kGy dose of γ-radiation.To understand how C. parvum avoids radiation damage, we characterized its protein expression patterns 6, 24, and 48 h after a 10-kGy dose of γ-radiation using two-dimensional PAGE. The gels showed 10 silver-stained spots that increased or decreased in size following γ-irradiation. Five proteins contained in these spots were identified using MALDI-TOF MS peptide fingerprinting, and two of these showed an increase in expression after γ-irradiation. These proteins were identified by LC–MS/MS as proteasome subunit alpha type 4 (NTN hydrolase fold) and thioredoxin peroxidase-like protein. The roles of these two upregulated proteins as related to the radioresistance of C. parvum remain to be evaluated. |
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Keywords: | Cryptosporidium parvum γ-Irradiation Proteasome Thioredoxin peroxidase |
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