Role of XIAP protein, a human member of the inhibitor of apoptosis (IAP) protein family, in phytohemagglutinin-induced apoptosis of human T cell lines |
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Authors: | I Vitté-Mony R G Korneluk F Diaz-Mitoma |
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Institution: | (1) Division of Virology, Children's Hospital of Eastern Ontario, Ontario, Canada;(2) Apoptogen, University of Ottawa, Ontario, Canada;(3) Department of Microbiology and Immunology, University of Ottawa, Ottawa Ontario, Canada |
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Abstract: | A new family of human genes xiap, hiap-1 and hiap-2, which are homologous to the baculovirus iap (inhibitor of apoptosis)
genes cp-iap and op-iap, has been recently cloned and shown to suppress apoptosis after serum withdrawal or exposure to a
free radical inducer. In order to examine the role of one of these human genes, namely xiap, in lymphoid cells, we studied
XIAP expression, after PHA stimulation in three different human T cell lines. We report here that stimulation with PHA resulted
in the human T cell lines undergoing apoptosis, as assessed by DNA fragmentation and by propidiumiodide (PI) staining and
flow cytometry. When XIAP protein expression was evaluated by Western blot, we observed that the induction of apoptosis by
PHA was associated with a parallel decrease of XIAP expression. We also provide evidence that stably transfected Jurkat cells
containing the xiap open reading frame became resistant to PHA-induced apoptosis. These data suggest a role for XIAP protein
in the regulation of apoptosis in lymphoid cells.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Apoptosis human T cells signal transduction |
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