| 盐爪爪Na^＋/H^＋逆向转运蛋白和焦磷酸酶的亚细胞定位 |
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| 引用本文： | 刘琳,张富春,曾幼玲.盐爪爪Na^＋/H^＋逆向转运蛋白和焦磷酸酶的亚细胞定位[J].西北植物学报,2009,29(3). |
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| 作者姓名： | 刘琳 张富春 曾幼玲 |
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| 作者单位： | 新疆大学,生命科学与技术学院,新疆生物资源基因工程重点实验室,乌鲁木齐,830046 |
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| 基金项目： | 新疆大学博士科研启动基金，新疆生物资源基因工程重点实验室开放项目 |
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| 摘 要： | 将盐爪爪Na+/H+逆向转运蛋白基因(KfNHX1)和焦磷酸酶基因(KfVP1)分别构建至植物表达载体,利用基因枪介导的方法转化洋葱表皮细胞,通过荧光显微镜观察研究其亚细胞定位.结果表明,转化了KfNHX1(或KfVP1)-GFP融合蛋白的洋葱表皮细胞仅膜系统散发荧光,而对照组即未转入KfNHX1(或KfVP1)基因的细胞则整体均匀发出荧光.说明KfNHX1和KfVP1可能定位于细胞的膜系统,作为跨膜转运蛋白在离子的调控运输中发挥重要作用.
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| 关 键 词： | 盐爪爪 绿色荧光蛋白 定位 |
Subcellular Localization of Na+/H+ Antiporter and H+-PPase of Kalidium foliatum |
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| LIU Lin,ZHANG Fuchun,ZENG You-ling.Subcellular Localization of Na+/H+ Antiporter and H+-PPase of Kalidium foliatum[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(3). |
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| Authors: | LIU Lin ZHANG Fuchun ZENG You-ling |
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| Institution: | Xinjiang Key Laboratory of Biological Resources and Genetic Engineering;College of Life Science and Technology;Xinjiang University;Urumqi 830046;China |
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| Abstract: | Kalidium foliatum Na+/H+antiporter and H+-PPase genes were separately constructed into the plant expression vector,and then transformed into onion epidermal cells by particle bombardment to study their subcellular localization.The results showed that green fluorescence was only visualized on the membrane system of onion epidermal cells transformed by pCAMBIA1301-1-KfNHX1(or KfVP1)-mGFP under fluorescence microscopy,whereas the controlled onion epidermal cells transformed by pCAMBIA1301-1-mGFP plasmid showed... |
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| Keywords: | NHX1 VP1 |
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