Tissue antioxidant status in streptozotocin-induced diabetes in rats |
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Authors: | Katherine H Thompson David V Godin Melvin Lee |
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Institution: | (1) Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, Canada;(2) Department of Pharmacology and Therapeutics, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada;(3) School of Family and Nutritional Sciences, University of British Columbia, Vancouver, British Columbia, Canada |
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Abstract: | Interactions between manganese (Mn) deficiency and streptozotocin (STZ)-diabetes with respect to tissue antioxidant status
were investigated in male, Sprague-Dawley rats. All rats were fed either a Mn-deficient (1 ppm) or a Mn-sufficient (45 ppm)
diet for 8 wk. Diabetes was then induced by tail-vein injection of STZ (60 mg/kg body weight), after which the rats were kept
for an additional 4 or 8 wk. The control groups comprised rats not injected with STZ and fed either Mn-deficient or Mn-sufficient
diets for a total of 12 wk.
The Mn-deficient diet decreased the activities of manganese superoxide dismutase (MnSOD) in kidney and heart, and of copperzinc
superoxide dismutase (CuZnSOD) in kidney, in the non-diabetic animals. In the diabetic rats, the Mn-deficient diet induced
more pronounced decreases in activities of these same enzymes, and also increased liver MnSOD activity. Plasma and hepatic
vitamin E levels increased progressively with the duration of diabetes, independent of dietary Mn intake. Lipid peroxidation,
as measured by H2O2-induced production of thiobarbituric acid reactive substances in erythrocytes, also increased, concomitant with decreased
liver and kidney glutathione (GSH) levels. These findings demonstrate for the first time an interactive effective between
Mn deficiency and STZ-diabetes, resulting in amplification of tissue antioxidant changes seen with either Mn deficiency or
STZ-diabetes alone. This effect of Mn deprivation in experimental diabetes suggests a physiological role for Mn as an antioxidant
nutrient. |
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Keywords: | Manganese deficiency and streptozotocin-diabetes MnSOD and diabetes CuZnSOD and diabetes tissue Mn status in diabetes mellitus Vitamin E and streptozotocin-diabetes STZ-diabetes and hepatic vitamin E SOD in Mn-deficient tissues |
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