Response of Sulfide:Quinone Oxidoreductase to Sulfide Exposure in the Echiuran Worm Urechis unicinctus |
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Authors: | Yu-Bin Ma Zhi-Feng Zhang Ming-Yu Shao Kyoung-Ho Kang Xiao-Li Shi Ying-Ping Dong Jin-Long Li |
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Institution: | (1) Ministry of Education, Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, 266003, China;(2) Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China;(3) Department of Aquaculture, Chonnam National University, Yeosu, 550749, South Korea; |
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Abstract: | Sulfide is a natural, widely distributed, poisonous substance, and sulfide:quinone oxidoreductase (SQR) is responsible for
the initial oxidation of sulfide in mitochondria. In this study, we examined the response of SQR to sulfide exposure (25,
50, and 150 μM) at mRNA, protein, and enzyme activity levels in the body wall and hindgut of the echiuran worm Urechis unicinctus, a benthic organism living in marine sediments. The results revealed SQR mRNA expression during sulfide exposure in the body
wall and hindgut increased in a time- and concentration-dependent manner that increased significantly at 12 h and continuously
increased with time. At the protein level, SQR expression in the two tissues showed a time-dependent relationship that increased
significantly at 12 h in 50 μM sulfide and 6 h in 150 μM, and then continued to increase with time while no significant increase
appeared after 25 μM sulfide exposure. SQR enzyme activity in both tissues increased significantly in a time-dependent manner
after 50 μM sulfide exposure. We concluded that SQR expression could be induced by sulfide exposure and that the two tissues
studied have dissimilar sulfide metabolic patterns. A U. unicinctus sulfide-induced detoxification mechanism was also discussed. |
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