Association of 1347 G/A cytochrome P450 4F2 (CYP4F2) gene variant with hypertension and stroke |
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Authors: | Anjana Munshi Vandana Sharma Subhash Kaul Amal Al-Hazzani Ali A Alshatwi Gowhar Shafi Rajeshwar Koppula Sai Babu Mallemoggala A Jyothy |
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Institution: | (1) Department of Molecular Biology, Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500016, India;(2) Department of Neurology, Nizams Institute of Medical Sciences, Punjagutta, Hyderabad, 500082, India;(3) Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia;(4) Dr. NTR University of Health Sciences, Vijayawada, Andhra Pradesh;(5) Department of Food Sciences and Nutrition, College of Food Sciences and Agriculture, King Saud University, Riyadh, Saudi Arabia |
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Abstract: | Genetic variants of cytochrome P450 4F2 (CYP4F2) gene have been suggested to be risk factors for hypertension, cardiovascular
diseases and stroke. In the present case–control study we investigated the association of 1347 G/A polymorphism (rs2108622)
in the 11th exon region of CYP4F2 gene with hypertension, ischemic stroke and stroke subtypes classified according to TOAST
(Trial of Org 10172 in Acute Stroke Treatment) classification. Five hundred and seven stroke patients (hypertensives: normotensives = 279:228)
and four hundred and eighty seven, age and sex matched controls (males: females = 356:131) (hypertensives: normotensives = 148:339)
were involved in the study. The genotypes were determined by PCR-RFLP technique. Genotypes were confirmed by subjecting the
PCR products to sequencing. Significant difference was observed in the genotypic distribution and allelic frequency between
the stroke patients and healthy controls. AA genotype and A allele associated significantly with stroke and hypertension P = 0.009; OR = 1.59 (95% CI = 1.119–2.283) and P = 0.010; OR = 1.26 (95% CI = 1.056–1.502); P = 0.01; OR = 1.58 (95% CI = 1.11–2.272) and P = 0.010; OR = 1.25(95% CI = 1.054–1.504) respectively]. A stepwise logistic regression analysis confirmed these findings.
To establish that this polymorphism is associated with stroke independent of hypertension; we compared stroke patients without
hypertension with normotensive controls. Significant difference was observed in genotypic distribution and allelic frequency
between the two groups (P = 0.001 and 0.002 respectively). Evaluating the association of this polymorphism with stroke subtypes we found significant
associations with cardioembolic stroke (P < 0.001). In conclusion our study suggests that 1347A allele of CYP4F2 gene is an important risk factor for hypertension
and ischemic stroke. |
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