Bmdsx基因在家蚕田岛嵌合体卵巢中的表达分析

根据雌特异性和雄特异性Bmdsx基因cDNA的共有序列,设计一对引物,以RT-PCR方法对家蚕田岛嵌合体蛹期卵巢中Bmdsx基因的表达情况进行了研究.结果表明,在家蚕田岛嵌合体蛹期卵巢中,在475bp附近和226bp附近分别扩增得到了一条条带,这两条条带大小与预期雌雄性别特异性Bmdsx基因cDNA片段大小相吻合.其中,雄特异性cDNA片段经DNA序列分析后得到了确认.

Analysis of Expression of Bmdsx Gene from the Ovary of Tashima Mosaic of Bombyx mori

Expression of Bmdsx gene from the ovary of the pupa of Tashima mosaic of Bombyx mori was studied by RT-PCR with a pair of primers based on the common cDNA fragments of male-specific and female-specific Bombyx mori doublesex gene.The result suggested that the two bands whose sizes were consistent with expected sizes of female-specific and male-specific Bombyx mori doublesex cDNA fragments were amplified near 475 bp and 226 bp respectively from the ovary of the pupa of Tashima mosaic of silkworm.The male-specific cDNA fragment Was confirmed by DNA sequence analysis.