虎鼬肾组织结构及水通道蛋白1,2 的表达

<正>虎鼬(Vormela peregusna)属哺乳纲(Mammalia)食肉目(Carnivora)鼬科(Mustelidae)虎鼬属(Vormela),是虎鼬属唯一一种小型哺乳动物,主要分布在东欧到中国西部的广大干燥地区,主要以啮齿动物为食(雷刚等,2009)。目前国内外有关虎鼬的研究比较少,包括核型分析(许可芬和高行宜,1986)、分子遗传学研究(Rozhnov et al.,

Light microscope structure of Vormela peregusna kidney and expression of AQP1,2 in kidney

Indices such as many kinds of blood inorganic ions and urea nitrogen were measured in this paper. The histological structure of kidney in Vormela peregusna was examined by using biological microscopy. The expression of Aquaporin1,2 (AQP1,2)was tested using immunohistochemical and stereological quantitative methods,and the expression intensity of Aquaporin1,2 (AQP1,2)was analyzed by using image pro-plus (IPP)image software. The results show that the concentration of K⁺ ,Na ⁺ ,Cl^- ,Ca ^{2 +} in serum of Vormela peregusna is higher than that in rat,however P ³⁺ is lower than that in rat.Creatinine,glucose and total cholesterol in serum also are higher than those in rat. Urea nitrogen is much higher than that in rat,and may be related to living in the Minqing Gansu desert and eating rodents that have high protein content. The degree of concentration of urine is high because the ratio of medulla and cortex is 3. 65∶ 1 and medulla is wide. AQP1 positive cells were expressed in the epithelium of proximal the convoluted tubule and thin segments of the medullary loop,and AQP2 was mainly expressed in epithelial cells of collecting ducts. Thus,AQP1,2 may play an important role water reabsorption by the kidney and the concentration of urine,which regulated by the water balance of kidney.