| DVS乳酸菌种菌株传代过程中遗传稳定性分析 |
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| 引用本文： | 刘衍芬,高学军,张明辉,葛增广.DVS乳酸菌种菌株传代过程中遗传稳定性分析[J].生物技术,2005,15(2):48-50. |
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| 作者姓名： | 刘衍芬 高学军 张明辉 葛增广 |
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| 作者单位： | 东北农业大学动物医学学院,黑龙江,哈尔滨,150030 |
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| 基金项目： | (2002年黑龙江省人事厅博士后基金项目(LRB00084) |
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| 摘 要： | 目的：对从直投式酸奶发酵剂中分离出的乳酸菌菌株传代过程中的遗传稳定性进行分析。方法：观察传代培养过程中各个菌株在转管不同次数时的形态变化，用RAPD方法分析传代过程中转管不同次数的乳酸菌基因组的变化趋势，并用SDSPAGE对比转管不同次数乳酸菌的蛋白质表达的差异。结果：乳球菌在传代过程中转管不同次数时基因组和蛋白质表达均无变化；乳杆菌1^#在传代过程中转管不同次数时基因组基本无变化，蛋白质表达有变化；乳杆菌2^#在传代过程中转管不同次数时基因组和蛋白质表达均有变化。结论：采用RAPD、SDS-PAGE方法可以有效地对直投式酸奶发酵剂菌株传代过程中的遗传稳定性进行分析。
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| 关 键 词： | DVS乳酸菌种 传代 遗传稳定性 RAPD SDS-PAGE |
| 文章编号： | 1004-311X(2005)02-0048-03 |
| 修稿时间： | 2004年8月31日 |
Genetic Stability Assessments of Lactic Acid Bacteria Separated from DVS during Subculture |
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| LIU Yan-fen,GAO Xue-jun,ZHANG Ming-hui,GE Zeng-guang.Genetic Stability Assessments of Lactic Acid Bacteria Separated from DVS during Subculture[J].Biotechnology,2005,15(2):48-50. |
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| Authors: | LIU Yan-fen GAO Xue-jun ZHANG Ming-hui GE Zeng-guang |
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| Institution: | LIU Yan-fen,GAO Xue-jun~*,ZHANG Ming-hui,GE Zeng-guang |
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| Abstract: | Objective:To analyse the genetic stability of lactic acid bacteria separated from DVS during subculture.Methods:Evaluate the change of each strain when transferred from one tube to another,evaluate the genome change of the different samples from different period during subculture using RAPD.SDS-PAGE was used to evaluate the change of protein expressing.Results:The genome and protein expressing of Streptococcus thermophillus held a good stability;Genome of Lactobacillus 1~# didn't change,but the protein of the whole cell changed a little while the genome and protein expressing of Lactobacillus 2~# both changed.Conclusions:The RAPD and SDS-PAGE technique therefore appear to be fast,simple and efficient method for evaluating genetic stability of lactic acid bacteria separated from DVS. |
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| Keywords: | Directed Vat Set subculture genetic stability RAPD SDS-PAGE |
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