Retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells is potentiated by leukemia inhibitory factor |
| |
Authors: | Pacherník J Bryja V Esner M Hampl A Dvorák P |
| |
Affiliation: | Laboratory of Molecular Embryology, Mendel University Brno, Zemedelská 1, 61300 Brno, Czech Republic. |
| |
Abstract: | Leukemia inhibitory factor (LIF) is a cytokine that exhibits proliferation, survival and differentiation in a wide range of cell types. Here we show that LIF potentiates retinoic acid-mediated neural induction in pluripotent P19 embryonal carcinoma cells. This activity of LIF was demonstrated by a profounded neural morphology followed by increased expression of neural-specific proteins (N-CAM, III beta-tubulin, and GAP-43), up-regulation of early neural lineage-specific gene Mash-1, and down-regulation of early endoderm-specific genes -fetoprotein and GATA-4. Moreover, LIF also slows growth and increases the level of apoptosis in differentiating cells. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|