Plant regeneration from protoplasts of Solanum species with potential agricultural value (S. hjertingii,S. polyadenium,S. capsicibaccatum) |
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| Authors: | Y -S Xu E Pehu R Malone M G K Jones |
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| Institution: | (1) Institute of Arable Crops Research, Rothamsted Experimental Station, 5 2JQ Harpenden, Herts AL, UK;(2) Present address: Department of Crop Husbandry, University of Helsinki, Helsinki, Finland;(3) Present address: Plant Sciences, School of Biological and Environmental Sciences, Murdoch University, 6150 Perth, W. Australia |
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| Abstract: | Protoplasts have been isolated from three tuber-bearing Solanum species, S. hjertingii, S. polyadenium and S. capsicibaccatum, that are sexually incompatible with S. tuberosum, but possess potentially useful characters. For isolating protoplasts from leaves of in vitro shoot cultures of S. hjertingii and S. capsicibaccatum growth was improved by including silver thiosulfate in the medium. However, for S. polyadenium, leaves of pot-grown plants were the best source for protoplasts. Following protoplast division and culture, plants were regenerated from protoplasts of each of the species. The pattern of chromosome variation in regenerants was similar to that observed for other diploid and tetraploid Solanum species. The results indicate that it should be possible to introduce the potentially useful germplasm from these wild species into somatic hybrids with S. tuberosum by protoplast fusion.Abbreviations STS
silver thiosulfate
- BAP
benzylaminopurine
- GA3
gibberellic acid
- NAA
naphthalene acetic acid
- IAA
indole-3-acetic acid |
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