The importance of proline on long-term hepatocyte function in a collagen gel sandwich configuration: regulation of protein secretion |
| |
Authors: | Lee J Morgan J R Tompkins R G Yarmush M L |
| |
Institution: | Surgical Services, Massachusetts General Hospital, Shriners Burns Institute, Boston, Massachusetts 02114. |
| |
Abstract: | A serum-free culture system for primary hepatocytes which maintains stabel high-level hepatocyte function for prolonged periods in culture has been developed. Isolated rat primary hepatocytes were cultured in serum-free media between two layer of gelled collagen in a sandwich configuration which reinstates the cellular polarity necessary for long-term function in vitro. Thsee serum-free hepatocyte cultures maintained near physiological rates of albumin and transferrin secretion for a minimum of 26 days in culture. L-Proline was shown to be critical for both the approach to steady state and maximal level of protein secretion. Analysis of does-response data gave K(m) values of 2.9 and 1.7 mug/mL for albumin and transferrin secretion, respectively. |
| |
Keywords: | albumin transferrin proline hepatocytes primary culture serum free long term |
本文献已被 PubMed 等数据库收录! |