cAMP,not Ca2+/calmodulin,regulates the phosphorylation of acetylcholine receptor in Torpedo californica electroplax |
| |
Authors: | George B Zavoico Carol Comerci Emily Subers John J Egan Chi-Kuang Huang Maurice B Feinstein Henry Smilowitz |
| |
Institution: | 1. Departments of Pharmacology, University of Connecticut Health Center, Farmington, CT 06032 U.S.A.;2. Departments of Oral Biology, University of Connecticut Health Center, Farmington, CT 06032 U.S.A.;3. Departments of Pathology, University of Connecticut Health Center, Farmington, CT 06032 U.S.A. |
| |
Abstract: | The regulation of the phosphorylation of the acetylcholine receptor in electroplax membranes from Torpedo californica and of purified acetylcholine receptor was investigated. The phosphorylation of the membrane-bound acetylcholine receptor was not stimulated by Ca2+/calmodulin, nor was it inhibited by EGTA, but it was stimulated by the catalytic subunit of cAMP-dependent protein kinase, and was blocked by the protein inhibitor of cAMP-dependent protein kinase. Purified acetylcholine receptor was not phosphorylated by Ca2+/calmodulin-dependent protein kinase activity in electroplax membranes, nor by partially purified Ca2+/calmodulin-dependent protein kinases from soluble or particulate fractions from the electroplax. Of the four acetylcholine receptor subunits, termed α, β, γ and δ, only the γ- and δ-subunits were phosphorylated by the cAMP-dependent protein kinase (+cAMP), or by its purified catalytic subunits. |
| |
Keywords: | Phosphorylation Calcium/calmodulin cyclic AMP dependence Protein kinase Acetylcholine receptor (T californica electroplax) Pipes 1 4-piperazinediethane-sulphonic acid |
本文献已被 ScienceDirect 等数据库收录! |
|