Identity of the Photosystem II reaction center polypeptide |
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| Authors: | HY Nakatani B Ke E Dolan CJ Arntzen |
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| Institution: | 1. MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824 U.S.A.;2. Charles F. Kettering Laboratory, Yellow Springs, OH 45387 U.S.A. |
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| Abstract: | A Photosystem-II (PS-II)-enriched chloroplast submembrane fraction has been subjected to non-denaturing gel-electrophoresis. Two chlorophyll a (Chl a)-binding proteins associated with the core complex were isolated and spectrally characterized. The Chl protein with apparent apoprotein mass of 47 kDa (CP47) displayed a 695 nm fluorescence emission maximum (77 K) and light-induced absorption characteristics indicating the presence of the reaction center Chl, P-680, and its primary electron acceptor, pheophytin. A Chl protein of apparent apoprotein mass of 43 kDa (CP43) displayed a fluorescence emission maximum at 685 nm. We conclude that CP43 serves as an antenna Chl protein and the PS II reaction center is located in CP47. |
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| Keywords: | Photosystem II Reaction center Fluorescence Chlorophyll-protein Pheophytin (Spinach) PS Photosystem Tricine LDS lithium dodecyl sulfate LHC monomeric form of LHC Pheo pheophytin Chl chlorophyll |
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