Calmodulin and the target size of the (Ca2+ + Mg2+)-ATPase of human red-cell ghosts |
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| Authors: | JoséD. Cavieres |
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| Affiliation: | Physiological Laboratory, University of Cambridge, Downing Street, Cambridge, CB2 3EG U.K. |
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| Abstract: | An average target size of 251 kDa has been obtained for the (Ca2+ + Mg2+)-ATPase of calmodulin-depleted erythrocyte ghosts by radiation inactivation with 16 MeV electrons. This is close to twice the size of the purified calcium-pump polypeptide. When calmodulin was included during the ATPase assay, a component of about 1 MDa appeared in addition to the activated dimer. |
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| Keywords: | Calmodulin Radiation inactivation (Erythrocyte membrane) Hepes, 4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid |
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