基因敲除动物的研究和应用

目的基因敲除动物是近十几年来发展起来的在个体水平上研究基因功能的一类实验动物,它以基因敲除技术和胚胎干细胞技术为基础,在生命科学研究的各个领域得到了广泛应用。最近两年发展起来的RNA干扰技术仍然不能代替它。本文综述了基因敲除动物在各医学生物领域的研究与应用、浅谈其与RNA干扰技术的比较及其发展前景。

Research and Application of Gene Knock-Out Animal Model

Objective Gene knock-out animal is a species of experiment animal which can let people research gene function at individual level. It is based on the gene knock-out technique and embryonic stem cells. Now, it is widely used in many fields of life science research. The gene knock-out technique can not be replaced by RNA interference technique which grew over the last few years. The study and application of gene knock-out animal were reviewed in this article. Comparison between gene knock-out technique and RNA interference technique was also overviewed.