Glycine and serine as tentative agonists for metabotropic glutamate receptors |
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Authors: | I I Abramets I V Komissarov I M Samoilovich Yu V Kuznetsov |
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Institution: | (1) Donetsk State Medical University, Ministry of Public Health of Ukraine, Ukraine |
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Abstract: | In experiments on slices of the rat hippocampus, glycine (Gly) and serine (Ser) in concentrations of 100 μM to I mM were found
to reversible increase the amplitudes of population EPSP (pEPSP) in pyramidal neurons of theCA1 hippocampal area, evoked by single electrical stimuli applied to Schaffer collaterals (SchC). This potentiation was not affected
by 100 μM of a non-competetive antagonist of NMDA glutamate receptors (GR), ketamine, but was considerably weakened by 500
μM of a competitive antagonist of metabotropic GR (mGR), (±)-4-carboxyphenylglycine (CFG). The effects of Gly and Ser were
not observed in the presence of 50 μM of a blocker of protein kinase C (PKC) catalytic subunit, polymixin B, but were not
modified by preliminary action on the slices of 10 μM of a calmodulin inhibitor, substance W-7. Gly and Ser also enhanced
long-term post-tetanic potentiation (LTPP) of synaptic transmission caused by high-frequency rhythmic stimulation of SchC.
Low-frequency (1/sec, 15 min) SchC stimulation abolished the potentiation of synaptic transmission evoked either by high-frequency
SchC stimulation or by the actions of Gly and Ser. The data allow us to suggest that Gly and Ser in millimolar concentrations
activate mGR, enhance relay functions of the synapses of pyramidal neurons in theCA1 hippocampal region, and facilitate plastic modifications in these synapses. |
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