| 尿微量白蛋白ELISA测定法最适条件的探讨 |
| |
| 引用本文： | 任凤琴,宋耀虹,冯涛.尿微量白蛋白ELISA测定法最适条件的探讨[J].生物化学与生物物理进展,1997,24(1):88-90. |
| |
| 作者姓名： | 任凤琴 宋耀虹 冯涛 |
| |
| 作者单位： | 中国医学科学院中国协和医科大学北京协和医院, 北京 100730;中国医学科学院中国协和医科大学北京协和医院, 北京 100730;中国医学科学院中国协和医科大学北京协和医院, 北京 100730 |
| |
| 摘 要： | 用自制兔抗人白蛋白抗体;酶标抗原和进口NUNC板, 进行了两种显色剂邻苯二胺(OPD)及四甲基联苯胺(TMB)测定尿微量白蛋白的比较,底物(H2O2)浓度对测定的影响,碳酸钠和戊二醛两种包被方法的比较,以及抗体和抗原浓度的选择等影响因素进行了实验探讨,并确定了本实验的最佳分析条件.
|
| 关 键 词： | 尿微量白蛋白，竞争酶联免疫吸附法，邻苯二胺 |
| 收稿时间： | 1996/7/11 0:00:00 |
| 修稿时间： | 1996/11/1 0:00:00 |
Studies on the Optimal Conditions for the Determination of Microalbumin in Urine by ELISA |
| |
| REN Fengqin,SONG Yaohong and FENG Tao.Studies on the Optimal Conditions for the Determination of Microalbumin in Urine by ELISA[J].Progress In Biochemistry and Biophysics,1997,24(1):88-90. |
| |
| Authors: | REN Fengqin SONG Yaohong and FENG Tao |
| |
| Institution: | Chinese Academy of Medical Sciences and Peking Union Medical College, Peking Union Medical College Hospital, Beijing 100730, China;Chinese Academy of Medical Sciences and Peking Union Medical College, Peking Union Medical College Hospital, Beijing 100730, China;Chinese Academy of Medical Sciences and Peking Union Medical College, Peking Union Medical College Hospital, Beijing 100730, China |
| |
| Abstract: | A solid phase and competitive enzyme immunoassay for the determination of microalbumin in urine using rabbit anti-human albumin antibody, HRP-HSA and Nunclon microtiter strips was developed. Two chromogens: o-phenylenediamine (OPD) and 3,3,5,5-tetremethyl benzidine (TMB), sodium carbonate coated wells and glutaraldehyde coated wells was compared. The influences on the determination of the concentration of substrated, antigen and antibody was discussed. The optimal conditions for the assay was established. |
| |
| Keywords: | urine microalbumin ELISA OPD |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
|
