MSCT评价兔VX2乳腺种植瘤抗血管生成治疗的研究

探讨多层螺旋CT（multi—slice spiral computed tomography,MSCT）灌注成像与肿瘤血管内皮生长因子（vascular endothelial growth factor,VEGF）表达的相关性以评估兔VX2乳腺种植瘤抗血管生成治疗的疗效。将69R乳腺VX：瘤兔于肿瘤生长2周后随机分为对照组（生理盐水1、恩度组（Endostar）、cEF组环磷酰胺（Cyclophosphamide C）、表阿霉素（EpirubicinE）和5-氟尿嘧啶（5．FluorouracilF）]、联合治疗CR（Endostar和CEF）。治疗2周后对瘤兔进行MSCT灌注扫描，获得血流量（bloodflow,BF）、血容量（bloodvolume，BV）、平均通过时间（meantransittime，MTT）及表面通透性（permeabilitysurface，PS）等灌注参数均值：随后取瘤组织进行免疫组化及Westernblot检测 VEGF蛋白表达情况。结果显示，对照组、CEF组、恩度组、联合治疗组BF、BV和Ps均与VEGF表达结果呈正相关（R对照组=0．896、0．680、0．765，RCEF组=0．877、0．876、0．852，R恩度组=0．804、0．924、0．888，R联合治疗组=0．780、0．735、0．744；P〈0．05），MTT均与VEGF表达结果呈负相关（R对照组＝-0．591，RCEF组=0．678，R恩度组=0．793，R联合治疗组=-0．687；P〈0．05）。MSCT灌注参数与VEGF蛋白表达具有相关性，MSCT灌注参数可以反映肿瘤治疗后免疫组化与分子水平VEGF表达的变化，MSCT可以在体无创评价兔VX2乳腺种植瘤抗血管生成治疗的疗效。

The Evaluation of Anti-angiogenic Treatment for Implanted Rabbit VX2 Breast Tumor Using Multi-slice Spiral Computed Tomography

The objective of this work was to investigate the correlation between multi-slice spiral computed tomography （MSCT） perfusion imaging and VEGF for assessing targeted anti-angiogenesis therapy on an implanted rabbit VX2 breast tumor model. 69 female rabbits were randomly assigned to one of the 4 groups and received treatment accordingly： control （saline）, Endostar, neoadjuvant chemotherapy （CEF） and combination therapy （Endostar and CEF）. After 2 weeks of treatment, MSCT perfusion scannings were performed for all rabbits and information about blood flow （BF）, blood volume （BV）, mean transit time （MTT） and surface permeability （PS） was collected. Then tumor tissue was dissected for immunohistochemistry and Western blot for VEGF expression.There was a positive correlation between VEGF expression and BE, BV, or PS level （P〈0.05） and a negative correlation between VEGF expression and MTT level for all 4 groups （P〈0.05）. Therefore, MSCT can be used as a non-invasive approach to evaluate the effect ofanti-angiogenic therapy for implanted rabbit VX2 breast tumors.