A simple method for enrichment of uninucleate conidia of Aspergillus oryzae |
| |
| Authors: | Hara Seiichi Tsuji Ryohei F Hatamoto Osamu Masuda Tsutomu |
| |
| Institution: | Noda Institute for Scientific Research, Noda-shi, Chiba, Japan. shara@mail.kikkoman.co.jp |
| |
| Abstract: | Aspergillus oryzae produces multinucleate conidia, which makes the obtaining of homokaryons labor-intensive. Analysis of conidia by flow cytometry clarified the relationship that conidia of lower nuclear number were smaller in size. Based on this, we have developed a simple way to enrich uninucleate conidia with a membrane filter. Our results also suggest that the method is useful for elimination of heterokaryons. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
