Regulation of mRNA levels of rat liver carbamoylphosphate synthetase by glucocorticosteroids and cyclic AMP as estimated with a specific cDNA |
| |
| Authors: | C J de Groot A J van Zonneveld P G Mooren D Zonneveld A van den Dool A J van den Bogaert W H Lamers A F Moorman R Charles |
| |
| Affiliation: | 1. Department of Anatomy and Embryology, University of Amsterdam Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands;1. Department of Molecular Biology, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, P.O. Box 9190, 1006 AD Amsterdam, The Netherlands |
| |
| Abstract: | The construction and cloning of a cDNA complementary to the mRNA of rat liver carbamoylphosphate synthetase (ammonia) is described. Using this cDNA, the size of the mature, cytosolic carbamoylphosphate synthetase (ammonia) mRNA is estimated to be 6.0 Kb. The levels of carbamoylphosphate synthetase (ammonia) mRNA in liver are shown to be regulated by glucocorticosteroids and cyclic AMP. By studying mRNA levels of carbamoylphosphate synthetase, albumin and phosphoenolpyruvate carboxykinase, using specific cDNA clones, we show that carbamoylphosphate synthetase gene expression, like that of albumin is liver-specific. |
| |
| Keywords: | CPS carbamoylphosphate synthetase PEPCK phosphoenolpyruvate carboxykinase pCPS preCPS ppALB preproalbumin TMV tobacco mosaic virus |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
