<Emphasis Type="Italic">myo</Emphasis>-Inositol Phosphate Isomers Generated by the Action of a Phytase from a Malaysian Waste-water Bacterium |
| |
| Authors: | Ralf Greiner Abd-Elaziem Farouk Nils-Gunnar Carlsson Ursula Konietzny |
| |
| Institution: | (1) Division of Bioprocess Engineering & Biotechnology, Centro Politécnico, Federal University of Paraná, Caixa Postal 19011, Jardim das Américas, Curitiba, PR, 81531-970, Brazil;(2) Biomolecular Engineering Research Unit, Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Jalan Gombak, 53100 Kuala Lumpur, Malaysia;(3) Department of Food Science, Chalmers University of Technology, 412 96 Gothenburg, Sweden |
| |
| Abstract: | Using a combination of High-Performance Ion Chromatography analysis and kinetic studies, the pathway of myo-inositol hexakisphosphate dephosphorylation by a phytase from a Malaysian waste-water bacterium was established. The data
demonstrate that the phytase preferably dephosphorylates myo-inositol hexakisphosphate in a stereospecific way by sequential removal of phosphate groups via D-I(1,2,3,4,5)P5, D-I(2,3,4,5)P4, D-I(2,3,4)P3, D-I(2,3)P2 to finally I(2)P. It was estimated that more than 90% of phytate hydrolysis occurs via D-I(1,2,3,4,5)P5. Thus, the phytase from the Malaysian waste-water bacterium has to be considered a 6-phytase (E.C. 3.1.3.26). A second pathway
of minor importance could be proposed which is in accordance with the results obtained from analysis of the dephosphorylation
products formed by the action of the phytase under investigation on myo-inositol hexakisphosphate. It proceeds via D/L-I(1,2,4,5,6)P5, D/L-I(1,2,4,5)P4, D/L-I(1,2,4)P3, D/L-I(2,4)P2 to finally I(2)P. |
| |
| Keywords: | myo-Inositol phosphate isomers Phytate-degrading enzyme Phytate Phytase Waste-water bacterium |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
