A combined HNCA/HNCO experiment for 15N labeled proteins with 13C at natural abundance期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

A combined HNCA/HNCO experiment for 15N labeled proteins with 13C at natural abundance

Authors:	Kupce Eriks Muhandiram D R Kay Lewis E

Affiliation:	(1) Varian Inc., Surrey, KT12 2QF, U.K;(2) Protein Engineering Network Centers of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, The University of Toronto, Toronto, Ontario, Canada, M5S 1A8

Abstract:	A triple resonance NMR experiment is presented for the simultaneous recording of HNCA and HNCO data sets on ¹⁵N, natural abundance ¹³C samples. The experiment exploits the fact that transfers of magnetization from ¹⁵N to ¹³CO and from ¹⁵N to ¹³C (and back) proceed independently for samples that are not enriched in ¹³C. A factor of 2 in measuring time is gained by recording the two data sets simultaneously with no compromise in spectral quality. An application to a 0.5 mM ¹⁵N labeled sample of protein-L is presented with all expected correlations observed in spectra recorded with a cryogenic probe at 500 MHz.

Keywords:	cryogenic probe HNCA HNCO ¹⁵N and natural abundance ¹³C protein samples triple resonance NMR
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