Clones Identification and Genetic Characterization of Garnacha Grapevine by Means of Different PCR-Derived Marker Systems |
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Authors: | Stefano Meneghetti Angelo Costacurta Enrica Frare Graziana Da Rold Daniele Migliaro Giacomo Morreale Manna Crespan Vicente Sotés Antonio Calò |
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Institution: | 1.C.R.A.-VIT Consiglio per la ricerca e la sperimentazione in agricoltura,Centro di ricerca per la viticoltura,Conegliano (TV),Italy;2.Universidad Politécnica de Madrid. ETSI Agrónomos, Ciudad Universitaria,Madrid,Spain |
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Abstract: | This study uses PCR-derived marker systems to investigate the extent and distribution of genetic variability of 53 Garnacha
accessions coming from Italy, France and Spain. The samples studied include 28 Italian accessions (named Tocai rosso in Vicenza
area; Alicante in Sicily and Elba island; Gamay perugino in Perugia province; Cannonau in Sardinia), 19 Spanish accessions
of different types (named Garnacha tinta, Garnacha blanca, Garnacha peluda, Garnacha roja, Garnacha erguida, Garnacha roya)
and 6 French accessions (named Grenache and Grenache noir). In order to verify the varietal identity of the samples, analyses
based on 14 simple sequence repeat (SSR) loci were performed. The presence of an additional allele at ISV3 locus (151 bp)
was found in four Tocai rosso accessions and in a Sardinian Cannonau clone, that are, incidentally, chimeras. In addition
to microsatellite analysis, intravarietal variability study was performed using AFLP, SAMPL and M-AFLP molecular markers.
AFLPs could discriminate among several Garnacha samples; SAMPLs allowed distinguishing few genotypes on the basis of their
geographic origin, whereas M-AFLPs revealed plant-specific markers, differentiating all accessions. Italian samples showed
the greatest variability among themselves, especially on the basis of their different provenance, while Spanish samples were
the most similar, in spite of their morphological diversity. |
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