| 猪肺炎支原体p97 C末端基因重组腺病毒的构建及其免疫效果 |
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| 引用本文： | 陈超,李媛,郭丹,曹培丽,张美晶,姜海芳,乔祖建,王亮,辛九庆.猪肺炎支原体p97 C末端基因重组腺病毒的构建及其免疫效果[J].微生物学报,2009,49(4):465-470. |
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| 作者姓名： | 陈超 李媛 郭丹 曹培丽 张美晶 姜海芳 乔祖建 王亮 辛九庆 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,动物细菌病研究室,哈尔滨150001 |
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| 基金项目： | 农业部院所长基金（NKLVBP200814） |
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| 摘 要： | 摘要：【目的】构建携猪肺炎支原体（Mycoplasma hyopneumoniae，Mhp）黏附因子p97 C末端基因的重组腺病毒并研究其诱导小鼠产生的免疫反应，为研制新型Mhp疫苗奠定基础。【方法】从Mhp基因组中扩增p97 C末端基因，并将其克隆到穿梭载体pShuttle-CMV中，该重组穿梭质粒经Pme I线性化后电转化到BJ5183-AD-1细胞中进行同源重组获得重组腺病毒DNA，纯化后的重组腺病毒质粒经Pac I酶切线性化后转染AD293细胞以获得重组腺病毒。对该重组腺病毒进行RT-PCR、间接
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| 关 键 词： | 关键词：猪肺炎支原体，猪支原体肺炎，p97基因，重组腺病毒，免疫效果 |
| 收稿时间： | 2008/10/15 0:00:00 |
| 修稿时间： | 1/1/2009 12:00:00 AM |
Construction of the recombinant adenovirus expressing the C-Terminal of the Mycoplasma hyopneumoniae p97 gene and its immune response |
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| Chao Chen,Yuan Li,Dan Guo,Peili Cao,Meijing Zhang,Haifang Jiang,Zujian Qiao,Liang Wang and Jiuqing Xin.Construction of the recombinant adenovirus expressing the C-Terminal of the Mycoplasma hyopneumoniae p97 gene and its immune response[J].Acta Microbiologica Sinica,2009,49(4):465-470. |
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| Authors: | Chao Chen Yuan Li Dan Guo Peili Cao Meijing Zhang Haifang Jiang Zujian Qiao Liang Wang and Jiuqing Xin |
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| Institution: | Division of Bacterial Disease, State Key laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001,China;Division of Bacterial Disease, State Key laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001,China;Division of Bacterial Disease, State Key laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001,China;Division of Bacterial Disease, State Key laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001,China;Division of Bacterial Disease, State Key laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001,China;Division of Bacterial Disease, State Key laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001,China;Division of Bacterial Disease, State Key laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001,China;Division of Bacterial Disease, State Key laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001,China;Division of Bacterial Disease, State Key laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001,China |
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| Abstract: | |
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| Keywords: | Keywords:Mycoplasma hyopneumoniae Mycoplasma pneumoniae of swine p97 gene Recombinant adenovirus Immune response |
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