Stably expressed <Emphasis Type="SmallCaps">d</Emphasis>-genome-derived HMW glutenin subunit genes transformed into different durum wheat genotypes change dough mixing properties |
| |
Authors: | A Gadaleta A E Blechl S Nguyen M F Cardone M Ventura J S Quick A Blanco |
| |
Institution: | (1) Department of Environmental and Agro-Forestry Biology and Chemistry, Section of Genetics and Plant Breeding, University of Bari, Via Amendola 165/A, 70126 Bari, Italy;(2) USDA-ARS Western Regional Research Center, Albany, CA 94710-1105, USA;(3) Dipartimento di Genetica e Microbiologia, University of Bari, Via Amendola 165/A, 70126 Bari, Italy;(4) Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523, USA |
| |
Abstract: | Durum wheat (Triticum turgidum L. var. durum) is traditionally used for the production of numerous types of pasta, and significant amounts are also used for bread-making,
particularly in southern Italy. The research reported here centres on the glutenin subunits 1Dx5 and 1Dy10 encoded by chromosome
1D, and whose presence in hexaploid wheats is positively correlated with higher dough strength. In order to study the effects
of stable expression of the 1Dx5 and 1Dy10 glutenin subunits in different durum wheat genotypes, four cultivars commonly grown
in the Mediterranean area (‘Svevo’, ‘Creso’, ‘Varano’ and ‘Latino’) were co-transformed, via particle bombardment of cultured
immature embryos, with the two wheat genes Glu-D1-1d and Glu-D1-2b encoding the glutenin subunits, and a third plasmid containing the bar gene as a selectable marker. Protein gel analyses of T1 generation seed extracts showed expression of one or both glutenin genes in four different transformed durum wheat plants.
One of these transgenic lines, DC2-65, showed co-suppression of all HMW-GS, including the endogenous ones. Transgene stability
in the transgenic lines has been studied over four generations (T1–T4). Fluorescence in situ hybridization (FISH) analysis of metaphase chromosomes from T4 plants showed that the integration of transgenes occurred in both telomeric and centromeric regions. The three plasmids were
found inserted at a single locus in two lines and in two loci on the same chromosome arm in one line. The fourth line had
two transgenic loci on different chromosomes: one with both glutenin plasmids and a different one containing only the construct
with the gene encoding the 1Dy10 glutenin subunit. Segregation of these two loci in subsequent generations allowed establishment
of two sublines, one containing both 1Dx5 and 1Dy10 and the other containing only 1Dy10. Small-scale quality tests showed
that accumulation of Dx5, Dy10 or both in transgenic durum wheat seeds resulted in doughs with stronger mixing characteristics.
A. Gadaleta and A. E. Blechl have contributed equally to this work. |
| |
Keywords: | Durum wheat Transformation Glutenin subunits Fluorescence in situ hybridization (FISH) |
本文献已被 SpringerLink 等数据库收录! |
|