| G_3基因工程菌发酵中pH值对包涵体形成及目的蛋白表达的影响 |
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| 引用本文： | 刘晓铭,林玉. G_3基因工程菌发酵中pH值对包涵体形成及目的蛋白表达的影响[J]. 生物技术, 1998, 0(5) |
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| 作者姓名： | 刘晓铭 林玉 |
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| 作者单位： | 广东汕头华茵生物工程公司!汕头515041 |
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| 摘 要： | 在不同pH值条件下摇瓶及罐发酵G3双体分子重组菌．探讨pH值对G3双体分子包涵体形成及目的蛋白表达水平的影响，确定G3双体分子重组菌发酵最佳pH值为生长期pH7．0—7．2，生产期pH6．7－6．8．
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| 关 键 词： | G_3双体分子 SDS—PAGE电泳 质粒 表达 |
pH INFLUENCE TO INCLUSION BODY AND PROTEIN IN G_3 FERMENTATION |
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| Liu Xiaoming, Lin Yu. pH INFLUENCE TO INCLUSION BODY AND PROTEIN IN G_3 FERMENTATION[J]. Biotechnology, 1998, 0(5) |
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| Authors: | Liu Xiaoming Lin Yu |
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| Affiliation: | Phargen Biotech Company Shantou S. E. Z.. Guangdong. 515041 |
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| Abstract: | We fermentated G3 recombination stain under different pH condition in flasks or fermenlor in order to find out the pH innuence, through this research we ascertained the optimum pH to growth period pH 7. 0-7. 2 and goal protein expression period pH 6. 7-6. 8 of G3 recombination stainand. |
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| Keywords: | G_3 SDS-PAGE electrophoresis plasmid expression |
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