Empirical evaluation of cellulase on enzymatic hydrolysis of waste office paper

Enzymatic hydrolysis of waste office paper was evaluated using three commercial cellulases, Acremonium cellulase, Meicelase, and Cellulosin T2. Varying the enzyme loading from 1 to 10% (w/w) conversion of waste office paper to reducing sugar was investigated. The conversion increased with the increase in the enzyme loading: in the case of enzyme loading of 10% (w/w), Acremonium cellulase yielded 79% conversion of waste office paper, which was 17% higher compared to Meicelase, 13% higher than that of Cellulosin T2. Empirical model for the conversion (%) of waste office paper to reducing sugar (x) was derived from experimental results as follow,x=kE ^m t ^(aE+b) wherek, m, a, and d denote empirical constants.E indicates initial enzyme concentration.