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27Al-NMR studies of aluminum transport across yeast cell membranes
Authors:Jagannatha Rao  KS  Easwaran  KRK
Institution:(1) Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore-, 570013, India;(2) Molecular Biophysics Unit, Indian Institute of Science, Bangalore-, 560 012, India
Abstract:Aluminum (Al) transport across yeast cells was studied using Dy(NO3)3 as a shift reagent by 27Al-NMR spectroscopy. The results showed that (a) Al enters the yeast cells at 15 min and over a period of time, within 4 h, an equilibrium sets in between outside and inside Al; (b) citrate does not favor Al going into the yeast cells at pH 5.0; and (c) EDTA brings out all the Al that has entered the yeast cells. (Mol Cell Biochem 175: 59–63, 1997)
Keywords:aluminum  yeast cells  transport  citrate  EDTA
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