Influence of bombardment with BA-coated microprojectiles on shoot organogenesis fromPhlox paniculata L. andPinus pinea L. tissues |
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| Authors: | Ill-Whan Sul Schuyler S Korban |
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| Institution: | (1) Department of Natural Resources and Environmental Science, 310 Madigan Building, University of Illinois, 1201 W. Gregory, 61801 Urbana, Illinois |
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| Abstract: | Summary Leaves ofPhlox paniculata L. were bombarded with BA-coated gold microprojectiles with the Biolistic PDS/1000-He gene gun. Increasing the concentration
of BA from 0.01 to 0.1 mg/μl increased the mean number of adventitious shoots from 7 to 16 shoots per leaf. Bombardment of
cotyledons ofPinus pinea L. (Italian stone pine) with BA-coated gold particles significantly reduced the frequency of shoot organogenesis by about
40%. However, induced shoot regenerants from bombarded explants of bothP. paniculata andP. pinea were longer (>1 cm) than those derived from control explants after 3 and 5 wk following bombardment, respectively. |
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| Keywords: | Phlox paniculata Pinus pinea organogenesis microprojectile bombardment cytokinin |
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