Kinesin 5B (KIF5B) Is Required for Progression through Female Meiosis and Proper Chromosomal Segregation in Mitotic Cells |
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| Authors: | Dawit Kidane Denny Sakkas Timothy Nottoli James McGrath Joann B. Sweasy |
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| Affiliation: | 1. Departments of Therapeutic Radiology and Genetics and The Yale Comprehensive Cancer Center, New Haven, Connecticut, United States of America.; 2. Yale Animal Genomics Services, Comparative Medicine Faculty, Yale School of Medicine, New Haven, Connecticut, United States of America.; 3. Departments of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut, United States of America.; University of Connecticut, United States of America, |
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| Abstract: | ![]()
The fidelity of chromosomal segregation during cell division is important to maintain chromosomal stability in order to prevent cancer and birth defects. Although several spindle-associated molecular motors have been shown to be essential for cell division, only a few chromosome arm-associated motors have been described. Here, we investigated the role of Kinesin 5b (Kif5b) during female mouse meiotic cell development and mitotic cell division. RNA interference (RNAi)-mediated silencing of Kif5b in mouse oocytes induced significant delay in germinal vesicle breakdown (GVBD) and failure in extrusion of the first polar body (PBE). In mitotic cells, knockdown of Kif5b leads to centrosome amplification and a chromosomal segregation defect. These data suggest that KIF5B is critical in suppressing chromosomal instability at the early stages of female meiotic cell development and mitotic cell division. |
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