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Straightforward Assay for Quantification of Social Avoidance in Drosophila melanogaster
Authors:Robert W. Fernandez  Marat Nurilov  Omar Feliciano  Ian S. McDonald  Anne F. Simon
Affiliation:1.Department of Molecular Biophysics and Biochemistry, Yale University;2.Department of Biology, York College/CUNY;3.Department of Biology, Western Ontario University
Abstract:
Drosophila melanogaster is an emerging model to study different aspects of social interactions. For example, flies avoid areas previously occupied by stressed conspecifics due to an odorant released during stress known as the Drosophila stress odorant (dSO). Through the use of the T-maze apparatus, one can quantify the avoidance of the dSO by responder flies in a very affordable and robust assay. Conditions necessary to obtain a strong performance are presented here. A stressful experience is necessary for the flies to emit dSO, as well as enough emitter flies to cause a robust avoidance response to the presence of dSO. Genetic background, but not their group size, strongly altered the avoidance of the dSO by the responder flies. Canton-S and Elwood display a higher performance in avoiding the dSO than Oregon and Samarkand strains. This behavioral assay will allow identification of mechanisms underlying this social behavior, and the assessment of the influence of genes and environmental conditions on both emission and avoidance of the dSO. Such an assay can be included in batteries of simple diagnostic tests used to identify social deficiencies of mutants or environmental conditions of interest.
Keywords:Neuroscience   Issue 94   social behavior   social avoidance   Drosophila melanogaster   Drosophila   Stress Odorant - dSO   T-maze apparatus   neurogenetics
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