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Synthesis of rosmarinic acid analogues in <Emphasis Type="Italic">Escherichia coli</Emphasis>

Authors:	Yibin Zhuang Jingjie Jiang Huiping Bi Hua Yin Shaowei Liu Tao Liu

Institution:	1.Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin,China;2.Department of Food Science and Technology, School of Biological Engineering,East China University of Science and Technology,Shanghai,China;3.Key Laboratory of Systems Microbial Biotechnology,Chinese Academy of Sciences,Tianjin,China

Abstract:	Objectives To produce rosmarinic acid analogues in the recombinant Escherichia coli BLRA1, harboring a 4-coumarate: CoA ligase from Arabidopsis thaliana (At4CL) and a rosmarinic acid synthase from Coleus blumei (CbRAS). Results Incubation of the recombinant E. coli strain BLRA1 with exogenously supplied phenyllactic acid (PL) and analogues as acceptor substrates, and coumaric acid and analogues as donor substrates led to production of 18 compounds, including 13 unnatural RA analogues. Conclusion This work demonstrates the viability of synthesizing a broad range of rosmarinic acid analogues in E. coli, and sheds new light on the substrate specificity of CbRAS.

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