Lithium-induced renal toxicity in rats: Protection by a novel antioxidant caffeic acid phenethyl ester |
| |
Authors: | Faruk Oktem Fehmi Ozguner Osman Sulak ?eref Olgar Onur Akturk H Ramazan Yilmaz Irfan Altuntas |
| |
Institution: | (1) Departments of Pediatric Nephrology, School of Medicine, Suleyman Demirel University, P.K. 13, 32100 Isparta, Turkey;(2) Departments of Physiology, School of Medicine, Suleyman Demirel University, Isparta, Turkey;(3) Departments of Anatomy, School of Medicine, Suleyman Demirel University, Isparta, Turkey;(4) Departments of Pediatrics, School of Medicine, Suleyman Demirel University, Isparta, Turkey;(5) Departments of Biochemistry, School of Medicine, Suleyman Demirel University, Isparta, Turkey;(6) Departments of Medical Biology and Genetics, School of Medicine, Suleyman Demirel University, Isparta, Turkey |
| |
Abstract: | Lithium carbonate used in the long-term treatment of manic-depressive illness has been reported to lead to progressive renal impairment in rats and humans. Caffeic acid phenethyl ester (CAPE), a component of honeybee propolis, protects tissues from reactive oxygene species mediated oxidative stress in ischemia-reperfusion and toxic injuries. The beneficial effect CAPE on lithium-induced nephrotoxicity has not been reported yet. The purpose of this study was to examine a possible renoprotective effect of CAPE against lithium-induced nephrotoxicity in a rat model. Twenty-two adult male rats were randomly divided into three experimental groups, as follows: control group, lithium-treated group (Li), and lithium plus CAPE-treated group (Li+CAPE). Li were treated intraperitoneally (i.p.) with 25 mg/kg Li2CO3 solution in 0.9% NaCl twice daily for 4 weeks. CAPE was co-administered i.p. with a dose of 10 μM/kg/day for 4 weeks. Serum Li, blood urea nitrogen and plasma creatinine, urinary N-acetyl-β-D-glucosaminidase (NAG, a marker of renal tubular injury), and malondialdehyde (MDA, an index of lipid peroxidation), were used as markers of oxidative stress-induced renal impairment in Li-treated rats. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities were studied to evaluate the changes of antioxidant status in renal tissue. Serum Li levels were found high in the Li and Li+CAPE groups. In Li-administrated rats, urinary NAG and renal MDA levels were increased according to control and Li+CAPE groups (p < 0.05). CAPE caused a significant reduction in the levels of these parameters. Likewise, renal SOD, CAT and GSH-Px activities were decreased in Li-administrated animals; CAPE caused a significant increase in the activities of these antioxidant enzymes. In conclusion, CAPE treatment has a protective effect against Li-induced renal tubular damage and oxidative stress in a rat model. |
| |
Keywords: | lithium caffeic acid phenethyl ester oxidative stress N-acetyl-β -D-glucosaminidase malondialdehyde nephrotoxicity |
本文献已被 PubMed SpringerLink 等数据库收录! |
|