| 微芯片电泳-紫外检测系统分析蛋白质 |
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| 引用本文： | 刘菁,庄贵生,徐良基,贾春平,金庆辉,赵建龙.微芯片电泳-紫外检测系统分析蛋白质[J].生物化学与生物物理进展,2004,31(11):1045-1049. |
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| 作者姓名： | 刘菁 庄贵生 徐良基 贾春平 金庆辉 赵建龙 |
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| 作者单位： | 1. 中国科学院上海微系统与信息技术研究所,上海,200050
2. 中国科学院上海微系统与信息技术研究所,上海,200050;生物芯片上海国家工程研究中心,上海,201203 |
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| 基金项目： | 国家高技术“863”计划项目（2002AA2Z2021）. |
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| 摘 要： | 微芯片电泳是基于微机电加工技术(MEMS)工艺，在芯片上的微管道中完成电泳检测过程的新型技术.依据紫外吸光度分析法，对蛋白质样品进行电泳分离与紫外检测.实验采用自控接口模板对进样及分离电压进行了系统的程序化控制，从而准确地控制整个电泳、检测流程，提高了微芯片电泳的分离效率和检测灵敏度.实验结果表明，夹流进样的方法可以有效分离混合蛋白，可用于蛋白质样品的分离检测.
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| 关 键 词： | 微芯片电泳，紫外检测，蛋白质 |
| 收稿时间： | 2004/6/15 0:00:00 |
| 修稿时间： | 2004/7/30 0:00:00 |
A Microchip Electrophoresis System With UV Detection for Protein Analysis |
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| LIU Jing,ZHUANG Gui-Sheng,XU Liang-Ji,JIA Chun-Ping,JIN Qing-Hui and ZHAO Jian-Long.A Microchip Electrophoresis System With UV Detection for Protein Analysis[J].Progress In Biochemistry and Biophysics,2004,31(11):1045-1049. |
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| Authors: | LIU Jing ZHUANG Gui-Sheng XU Liang-Ji JIA Chun-Ping JIN Qing-Hui and ZHAO Jian-Long |
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| Institution: | Shanghai Institute of Microsystem and Information Technology, The Chinese Academy of Sciences,Shanghai 200050, China;Shanghai Institute of Microsystem and Information Technology, The Chinese Academy of Sciences,Shanghai 200050, China;Shanghai Institute of Microsystem and Information Technology, The Chinese Academy of Sciences,Shanghai 200050, China;Shanghai Institute of Microsystem and Information Technology, The Chinese Academy of Sciences,Shanghai 200050, China;Shanghai Institute of Microsystem and Information Technology, The Chinese Academy of Sciences,Shanghai 200050, China;Shanghai Institute of Microsystem and Information Technology, The Chinese Academy of Sciences,Shanghai 200050, China;National Engineering Research Center For Shanghai Biochip Technology, Shanghai 201203, China |
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| Abstract: | The electrophoretic microchip fabricated by MEMS technology is successfully used to finish the electrophoresis process. The albumen-detected technology is based on the UV- absorption principle. The utilization of the procedural control makes the experimental process convenient and exact, and therefore improves the separation efficiency and detection sensitivity. It is proved that the pinched injection can separate the mixed proteins efficiently in this on-chip capillary. |
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| Keywords: | microchip electrophoresis UV detection protein |
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