| Detection and Analysis of an Estrous－associated Oviductal Glycoprotein DNA Fragment from Primates by PCR |
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| 引用本文： | 陈清轩.Detection and Analysis of an Estrous－associated Oviductal Glycoprotein DNA Fragment from Primates by PCR[J].基因组蛋白质组与生物信息学报,1995(1). |
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| 作者姓名： | 陈清轩 |
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| 作者单位： | CHEN Qing-xuan; Clayton E. Walton; Colin D. Nancarrow(Institute of Developmental Biology，Chinese Academy of Sciences，Beijing 100080)(CSIRO Division of Animal Production，Blacktown，N. S. W.2148，Australia) |
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| 摘 要： | ＤｅｔｅｃｔｉｏｎａｎｄＡｎａｌｙｓｉｓｏｆａｎＥｓｔｒｏｕｓ－ａｓｓｏｃｉａｔｅｄＯｖｉｄｕｃｔａｌＧｌｙｃｏｐｒｏｔｅｉｎＤＮＡＦｒａｇｍｅｎｔｆｒｏｍＰｒｉｍａｔｅｓｂｙＰＣＲ￥ＣＨＥＮＱｉｎｇ－ｘｕａｎ（陈清轩）；ＣｌａｙｔｏｎＥ．Ｗａｌｔｏ...
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Detection and Analysis of an Estrous-associated Oviductal Glycoprotein DNA Fragment from Primates by PCR |
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| CHEN Qing-xuan, Clayton E. Walton, Colin D. Nancarrow.Detection and Analysis of an Estrous-associated Oviductal Glycoprotein DNA Fragment from Primates by PCR[J].Genomics Proteomics & Bioinformatics,1995(1). |
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| Authors: | CHEN Qing-xuan Clayton E Walton Colin D Nancarrow |
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| Abstract: | DNA was extracted from lymphocytes of primates and PCR was used to amplify the estrous-associated glycoprotein DNA fragment. 537 kb DNA fragments from crab eating macaque were detected. Sequences from chimpanzee, orangutan and human were also amplified, but they were shorter than predicted. We were unable to amplify sequences from spider monkey, salmon, several rodents, bandicoot, sheep and pig . Analysis of the restriction map revealed several conserved RE sites in the amplified sequences of the primates. The results support the view that monkey has more close relationship with human than any other species at molecular level. |
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| Keywords: | DNA amplification PCR Primates Restriction map |
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