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Cloning and sequencing the HinfI restriction and modification genes |
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| Authors: | Srinivasan Chandrasegaran Keith D. Lunnen Hamilton O. Smith Geoffrey G. Wilson |
| Affiliation: | a Department of Environmental Health Sciences, The Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205, U.S.A. Tel. (301)955-0023 b New England Biolabs, Inc., Beverly, MA 01915, U.S.A. Tel. (508)927-5054 c Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A. |
| Abstract: | The HinfI restriction and modification genes were cloned on a 3.9-kb PstI fragment inserted into the PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb BclI-AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa). |
| Keywords: | Haemophilus influenzae Escherichia coli recombinant DNA plasmids pBR322 and pUC19 restriction endonuclease methylase |
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