Somatic embryogenesis from leaf and zygotic embryo explants of Blighia sapida ‘Cheese’ ackee |
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Authors: | S A Webster S A Mitchell W A Reid M H Ahmad |
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Institution: | (1) Biotechnology Centre, The University of the West Indies, Mona Campus, Kingston 7, Jamaica;(2) Electronic Microscopy Unit, The University of the West Indies, Mona Campus, Kingston 7, Jamaica |
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Abstract: | Summary This study investigated factors affecting the production of somatic embryos in Blighia sapida (ackee). Explants obtained from fully expanded leaves or cotyledons of immature zygotic embryos excised from brown (BSCZE)
or green seeds (GSCZE) were cultured on Murashige and Skoog medium supplemented with 9, 18 and 36μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 4.4 or 22.1 μM benzylaminopurine (BAP) or 0.2–19.9 μM thidiazuron (TDZ). Leaf explants grown on media supplemented with the different combinations of 2,4-D and BAP formed callus,
but they were non-embryogenic, while explants were not responsive on TDZ-supplemented media. GSCZE explants grown in the presence
of 2,4-D/BAP combinations of 9/4.4, 18/4.4 or 36/4.4 μM formed non-embryogenic callus profusely, but explants gave rise to organized globular protuberances (GPs) and non-embryogenic
callus on media containing TDZ, with the best concentration at 0.4 μM. BSCZE explants grown on TDZ-supplemented media also formed callus, but no GPs were detected. When GPs were cultured on media
containing TDZ and abscisic acid they (ABA), gave rise to the highest number of somatic embryos. The medium was also beneficial
for the development of somatic embryos from the globular to cotyledonary stage. |
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Keywords: | cotyledon immature zygotic embryo thidiazuron |
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