花生种子萌发过程中胚轴多胺氧化酶的活性变化

研究花生(Arachis hypogaea)种子萌发过程中胚轴多胺氧化酶(PAO)的活性变化及其与种子萌发的关系表明：胚轴中的PAO活性是在种子萌发过程中逐渐形成的，而黑暗条件更有利于该酶的活性形成；放线菌素D(10mg／L)、环己酰亚胺(10mg／L)处理对种子萌发的抑制率分别为26.3％和87.3％，对胚轴PAO活性的抑制率分别为41.1％和94.0％，显示胚轴中的PAO很可能参与花生种子的萌发过程，且其mRNA在种子发育过程中已合成并贮存于种子中，萌发时PAO活性的出现主要是由于这些mRNA转译合成了PAO。

The Changes of Polyamine Oxidase Activity in Embryonic Axes During Peanut Seed Germination

The activity of polyamine oxidase in embryonic axes during peanut (Arachis hypogaea ) seed germination was investigated. Peanut seeds were soaked for 12 h and allowed to germinate at 30℃ in moistened vermiculite. The PAO activity did not appear within the first 12 h of germination, but occurred when the germination time was 24 h and increased from then on. The PAO activity was higher when the peanut seeds germinated in the dark than in the light (1600 Lx). When the peanut seeds were treated with 10 mg/L actinomycin D (Act-D), a RNA sunthesis inhibitor or cycloheximide (CHM), a protein synthesis inhibitor for 96 h, the inhibitions of germination and PAO activity were 26.3% and 41.1% by Act-D or 87.3% and 94.0% by CHM. All of these results suggested that PAO was involved in the peanut seed germination.