Studies on the 5α-androstane-3β, 17β-diol-6α-hydroxylase and on the 5α-androstane-3β, 17β-diol-7α-hydroxylase in anterior hypophysis of male rats. |
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| Authors: | Jean-Michel Guiraud Suzanne Samperez Pierre Jouan |
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| Affiliation: | Laboratoire de Biochimie des Hormones (ERA-CNRS, 567) Centre Régional de Recherche en Endocrinologie 2, Avenue du Professeur L. Bernard 35043 RENNES, Cedex, FRANCE |
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| Abstract: | In anterior pituitaries from male rats, it appeared that 5α-androstane-3β, 17β-diol was quickly metabolized into 5α-androstane-3β,6α-17β-triol and 5α-androstane-3β,7α, 17β-triol by action of 6α- and 7α-hydroxylases. Hydroxysteroid hydroxylases were located in endoplasmic reticulum and were dependent on NADPH+. Their optimum pH was 8.0, optima temperature, 37°C, and their apparent Km was 2.7 μM. Hydroxylative reactions were not reversible and not modified by gonadectomy. Hydroxylation seemed an efficient control of the pituitary level of 5α-andros-tane-3β, 17β-diol. |
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| Keywords: | Cholesterol : 5-cholesten-3β -ol. Progesterone : 4-pregnene-3 20-dione. Androsterone : 3α -hydroxy-5α -androstan-17-one. Estrone : 3-hydroxy-1 3 5(10)-estra-trien-17-one. Estriol : 1 3 5(10)-estratriene-3 16α 17β -triol. 3 6 17-Trione : 5α -androstane-3 6 17-trione |
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