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| 表达小反刍兽疫H蛋白的重组山羊痘病毒疫苗 | |
| 引用本文： | 陈伟业,曲林茂,胡森,胡倩倩,张倩,支海兵,黄克和,步志高.表达小反刍兽疫H蛋白的重组山羊痘病毒疫苗[J].生物工程学报,2009,25(4):496-502. |
| 作者姓名： | 陈伟业 曲林茂 胡森 胡倩倩 张倩 支海兵 黄克和 步志高 |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,农业部兽医公共卫生重点开放实验室,哈尔滨,150001 2. 南京农业大学动物医学院,南京,210095 3. 中国兽医药品监察所,北京,100081 |
| 基金项目： | 国家高技术研究发展计划(863计划)(No.20060102Z449)资助~~ |
| 摘 要： | 本研究旨在构建表达小反刍兽疫H蛋白的重组山羊痘病毒,并评价其免疫效力。利用筛选基因gpt和eGFP并结合空斑技术,纯化筛选了重组小反刍兽疫H基因的重组山羊痘病毒(rGPV-PPRV-H),经过PCR鉴定重组病毒已纯化。免疫荧光和蛋白印迹都表明重组病毒能够感染绵羊羔羊睾丸细胞表达小反刍兽疫H蛋白。以2×106PFU的rGPV-PPRV-H皮内注射免疫山羊6只,并于首次免疫后28d以相同剂量进行二次免疫。免疫后采血分离血清进行病毒中和试验,结果表明,一次免疫后21d,山羊痘病毒中和抗体效价依次为40、80、≥80、≥80、40、≥80,和小反刍兽疫病毒中和抗体效价全部转阳依次为80、80、80、80、40、40、10;二次免疫后14d,山羊痘病毒中和抗体效价抗体全部大于或等于80,小反刍兽疫病毒中和抗体效价依次为≥80、80、≥80、80、80、40,应该具有对山羊痘病毒和小反刍兽疫病毒强毒攻击的完全免疫保护作用。本研究为小反刍兽疫重组山羊痘疫苗的产业化提供了参考。 |
| 关 键 词： | 重组疫苗 小反刍兽疫 山羊痘病毒载体 中和抗体 |
| 收稿时间： | 2008/11/14 0:00:00 |
Recombinant goat pox virus expressing PPRV H protein |
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| Weiye Chen,Linmao Qu,Sen Hu,Qianqian Hu,Qian Zhang,Haibing Zhi,Kehe Huang and Zhigao Bu.Recombinant goat pox virus expressing PPRV H protein[J].Chinese Journal of Biotechnology,2009,25(4):496-502. | |
| Authors: | Weiye Chen Linmao Qu Sen Hu Qianqian Hu Qian Zhang Haibing Zhi Kehe Huang and Zhigao Bu |
| Institution: | State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Instituteof Chinese Academy of Agricultural Sciences, Key Laboratory of Veterinary Public Health Ministry of Agriculture, Harbin 150001, China;College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Instituteof Chinese Academy of Agricultural Sciences, Key Laboratory of Veterinary Public Health Ministry of Agriculture, Harbin 150001, China;College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Instituteof Chinese Academy of Agricultural Sciences, Key Laboratory of Veterinary Public Health Ministry of Agriculture, Harbin 150001, China;China Institute of Veterinary Drug Control, Beijing 100081, China;College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Instituteof Chinese Academy of Agricultural Sciences, Key Laboratory of Veterinary Public Health Ministry of Agriculture, Harbin 150001, China |
| Abstract: | The purpose of the study is to construct recombinant goat pox virus (GPV) expressing Peste des petits ruminants virus (PPRV) H protein,and to evaluate the immunization effect. Recombinant GPV containing PPRV H gene (rGPV-PPRV-H) was selected and purified by gpt and eGFP utilizing plaque purification,and the final selected recombinant GPV was proved to be purified by PCR. Immunofluorescence and Western blotting showed that the recombinant virus could express H protein of PPRV while infecting lamb testis cell... |
| Keywords: | recombinant vaccine Peste des petits of ruminants (PPR) goat pox virus vector neutralization antibodies |
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