Ciona intestinalis NADH dehydrogenase NDX confers stress-resistance and extended lifespan on Drosophila |
| |
| Authors: | Dmytro V Gospodaryov Oleh V Lushchak Bohdana M Rovenko Natalia V Perkhulyn Mike Gerards Tea Tuomela Howard T Jacobs |
| |
| Institution: | 1. BioMediTech and Tampere University Hospital, University of Tampere, FI-33014, Finland;2. Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk 76025, Ukraine;3. Research Program of Molecular Neurology, University of Helsinki, FI-00014, Finland |
| |
| Abstract: | An assembled cDNA coding for the putative single-subunit NADH dehydrogenase (NDX) of Ciona intestinalis was introduced into Drosophila melanogaster. The encoded protein was found to localize to mitochondria and to confer rotenone-insensitive substrate oxidation in organello. Transgenic flies exhibited increased resistance to menadione, starvation and temperature stress, and manifested a sex and diet-dependent increase in mean lifespan of 20–50%. However, NDX was able only weakly to complement the phenotypes produced by the knockdown of complex I subunits. |
| |
| Keywords: | Alternative respiratory chain enzymes Tunicate Gene therapy Oxidative stress Ageing |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
