| 荷兰鸢尾(Iris xiphium L. var. hybridum)的组织培养 |
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| 引用本文： | 黄苏珍,谢明云,佟海英,韩玉林,顾姻,居丽.荷兰鸢尾(Iris xiphium L. var. hybridum)的组织培养[J].植物资源与环境学报,1999(3). |
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| 作者姓名： | 黄苏珍 谢明云 佟海英 韩玉林 顾姻 居丽 |
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| 作者单位： | 江苏省中国科学院植物研究所，南京市玄武湖公园 |
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| 摘 要： | 取荷兰鸢尾（ＩｒｉｓｘｉｐｈｉｕｍＬ．ｖａｒ．ｈｙｂｒｉｄｕｍ）鳞茎片不同部位外植体块，接种于附加不同激素配比的基本培养基上，其中取自鳞茎片基部外植体块２ｍｍ×２ｍｍ×２ｍｍ，培养基为ＭＳ＋ＢＡ１．０ｍｇ／Ｌ＋ＮＡＡ０．２ｍｇ／Ｌ的不定芽诱导率为最高（７０％）；最理想的增殖培养基为ＭＳ＋ＢＡ２．０ｍｇ／Ｌ＋ＮＡＡ０．２ｍｇ／Ｌ。不定芽直径４～５ｍｍ，培养基为ＭＳ＋ＢＡ０．２ｍｇ／Ｌ＋ＮＡＡ０．５ｍｇ／Ｌ有利于不定根的发生，诱导生根率达８３３％。试管苗不经练苗可直接出瓶，移栽于泥炭∶田土∶河沙＝１∶１∶１（Ｖ／Ｖ）的基质中。
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| 关 键 词： | 荷兰鸢尾 组织培养 鳞片 |
The tissue culture of Iris xiphium L. var. hybridum |
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| \ Huang Suzhen,Xie Mingyun,Tong Haiyin,Han Yulin,Gu Yin.The tissue culture of Iris xiphium L. var. hybridum[J].Journal of Plant Resources and Environment,1999(3). |
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| Authors: | \ Huang Suzhen Xie Mingyun Tong Haiyin Han Yulin Gu Yin |
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| Abstract: | The bulb segments of Iris
xiphium L. var. hybridum were cultured on MS medium supplemented with variable ratio of
hormones. The results showed that the adventitious buds were easily proliferated from the
segments taken from the lower part of the bulb, the highest induction frequency (70%) was
obtained on MS medium supplemented with BA 1.0 mg/L NAA 0.2 mg/L, and MS BA 2.0
mg/L NAA 0.2 mg/L is most ideal proliferated medium; while MS medium supplemented with BA
0.2 mg/L NAA 0.5 mg/L is most effective (83.3%) for rooting. Rooting plantlets were easily to be
planted on a mixed medium of 1/3 peat 1/3 soil 1/3 sand (V/V) without any training. |
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| Keywords: | Iris xiphium L var hybridum tissue culture scale |
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