A rapid method for counting cell nuclei using a particle sizer/counter |
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| Authors: | Andy A Lin Thang Nguyen William M Miller |
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| Institution: | (1) Chemical Engineering Department, Northwestern University, 60208-3120 Evanston, IL, USA |
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| Abstract: | Summary We report here an improved method for nuclei counting utilizing Triton-X 100 to reduce the size of cell debris, thereby allowing the use of a particle sizer/counter. Furthermore, nuclei are completely released within 30 seconds, as compared to 1 hour using hypotonic solution. The method is accurate above 0.3 × 106 cells/mL. |
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