Effect of mesenchymal stromal cells encapsulated within polyethylene glycol-collagen hydrogels formed in situ on alkali-burned corneas in an ex vivo organ culture model |
| |
| Authors: | Kyung-Sun Na Gabriella Maria Fernandes-Cunha Ignacio Blanco Varela Hyun Jong Lee Youngyoon Amy Seo David Myung |
| |
| Institution: | 1. Department of Ophthalmology, Yeouido St. Mary''s Hospital, College of Medicine, The Catholic University of Korea, Seoul, South Korea;2. Department of Ophthalmology, Byers Eye Institute, Stanford University School of Medicine, Palo Alto, California, USA;3. Department of Chemical Engineering, Stanford University, Palo Alto, California, USA;4. Department of Chemical and Biological Engineering, Gachon University, Seongnam‐si, South Korea;5. VA Palo Alto HealthCare System, Palo Alto, California, USA;1. Institute for Regenerative Medicine, University of Zurich, Zurich, Switzerland;2. Center for Applied Biotechnology and Molecular Medicine, Zurich, Switzerland;3. La Colline Research Fellow, La Colline, Sion, Switzerland;4. Musculoskeletal Research Unit, VetSuisse Faculty, University of Zurich, Zurich, Switzerland;1. Department of Endocrinology, First Medical Center of People''s Liberation Army General Hospital, Beijing, China;2. Department of Endocrinology, Beijing Rehabilitation Hospital of Capital Medical University, Beijing, China;3. Department of Molecular Biology, Institute of Basic Medicine, School of Life Science, People''s Liberation Army General Hospital, Beijing, China;1. Department of Hematology and Cell Therapy, Oran, Algeria;2. European Society for Blood and Marrow Transplantation Global Committee, Paris, France;3. Institut Paoli Calmettes, Cancer Research Center of Marseille, Aix Marseille University, Marseille, France;4. Department of Hematology and Cell Therapy, Centre Hospitalier Universitaire, Nantes, France;5. University College London Cancer Institute, London, UK;6. Department of Hematology and Cell Therapy, Hospices Civils, Lyon, France;7. Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice, Poland;8. University of Freiburg, Freiburg, Germany;9. Policlinico Universitario Tor Vergata, Rome, Italy;10. Department of Hematology and Stem Cell Transplantation, Centre Hospitalier Universitaire, Bordeaux, France;11. Department of Hematology and Cell Therapy, Hôpital Saint-Antoine, Sorbonne University, Paris, France |
| |
| Abstract: | Background aimsCorneal inflammation after alkali burns often results in vision loss due to corneal opacification and neovascularization. Mesenchymal stem cells (MSCs) and their secreted factors (secretome) have been studied for their anti-inflammatory and anti-angiogenic properties with encouraging results. However, topical instillation of MSCs or their secretome is often accompanied by issues related to delivery or rapid washout. Polyethylene glycol (PEG) and collagen are well-known biomaterials used extensively in scaffolds for tissue engineering. To effectively suppress alkaline burn-induced corneal injury, the authors proposed encapsulating MSCs within collagen gels cross-linked with multi-functional PEG-succinimidyl esters as a means to deliver the secretome of immobilized MSCs.MethodsHuman MSCs were added to a neutralized collagen solution and mixed with a solution of four-arm PEG-N-hydroxysuccinimide. An ex vivo organ culture was conducted using rabbit corneas injured by alkali burn. MSCs were encapsulated within PEG-collagen hydrogels and injected onto the wounded cornea immediately following alkali burn and washing. Photographs of the ocular surface were taken over a period of 7 days after the alkali burn and processed for immunohistochemical evaluation. Samples were split into three groups: injury without treatment, MSCs alone, and MSCs encapsulated within PEG-collagen hydrogels.ResultsAll corneas in ex vivo organ culture lost their transparency immediately after alkali burn, and only the groups treated with MSCs and MSCs encapsulated within PEG-collagen hydrogels recovered some transparency after 7 days. Immunohistochemical analysis revealed increased expression of vimentin in the anterior corneal stroma of the group without treatment indicative of fibrotic healing, whereas less stromal vimentin was detected in the group containing MSCs encapsulated within the PEG-collagen hydrogels.ConclusionsPEG-collagen hydrogels enable the encapsulation of viable MSCs capable of releasing secreted factors onto the ocular surface. Encapsulating MSCs within PEG-collagen hydrogels may be a promising method for delivering their therapeutic benefits in cases of ocular inflammatory diseases, such as alkali burn injuries. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
