Isolation of somatic hybrids by cloning Nicotiana heterokaryons in nurse cultures |
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| Authors: | L. Menczel G. Lázár P. Maliga |
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| Affiliation: | (1) Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, Szeged, P.O. Box 521, Szeged, Hungary |
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| Abstract: | Fusion of Nicotiana knightiana Goodsp. and kanamycin resistant Nicotiana sylvestris Speg. et Com. protoplasts was induced by polyethylene glycol treatment. Heterokaryons were isolated by micropipette and transferred to nurse cultures of albino cells. Colonies originating from the heterokaryons could subsequently be distinguished by their green colour. The somatic hybrid nature of four such colonies was confirmed by isoenzyme pattern, kanamycin resistance and restored morphogenic potential. An additional kanamycin resistant line with characteristic Nicotiana knightiana isoenzymes was also found indicating that the drug resistance in the kanamycin resistant parent is under cytoplasmic control.Abbreviations ADH alcohol dehydrogenase - Nk Nicotiana knightiana |
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| Keywords: | Hybrids (somatic) Isoenzymes Kanamycin resistance Nicotiana Somatic hybrids |
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