| 新疆胀果甘草内生菌的分离和鉴定 |
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| 引用本文： | 宋素琴,欧提库尔·玛合木提,张志东,唐琦勇.新疆胀果甘草内生菌的分离和鉴定[J].微生物学通报,2007,34(5):0867-0870. |
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| 作者姓名： | 宋素琴 欧提库尔·玛合木提 张志东 唐琦勇 |
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| 作者单位： | 新疆农业科学院微生物应用研究所,乌鲁木齐,830091 |
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| 基金项目： | 新疆维吾尔自治区新疆特殊环境微生物重点实验室开放课题 |
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| 摘 要： | 对采自新疆的健康野生胀果甘草不同组织中的内生菌进行分离,确立了分离的条件为5%NaClO4浸5min,分离纯化得到149株细菌和2种真菌。通过形态学观察和革兰氏染色,细菌中芽孢杆菌为93株,杆状菌56株,使用法国梅里埃细菌自动鉴定仪对其进行鉴定,得到鉴定结果的细菌属于13个属,真菌显微形态鉴定属于Penicillium青霉菌属和Fusarium镰刀菌属。
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| 关 键 词： | 内生菌 胀果甘草 健康甘草组织 |
| 文章编号： | 0253-2654（2007）05-0867-04 |
| 修稿时间： | 2007-01-23 |
Isolation and Characterization of Endophytic Microorganisms in Glaycyrrhiza inflat Bat. from Xinjiang |
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| SONG Su-Qin,OTKUR Mahmut,ZHANG Zhi-Dong and TANG Qi-Yong.Isolation and Characterization of Endophytic Microorganisms in Glaycyrrhiza inflat Bat. from Xinjiang[J].Microbiology,2007,34(5):0867-0870. |
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| Authors: | SONG Su-Qin OTKUR Mahmut ZHANG Zhi-Dong and TANG Qi-Yong |
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| Institution: | Institute of Micobiology, Xinfiang Academy of Agricultural Sciences, Ururnqi 830091 |
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| Abstract: | The present investigation was undertaken in order to select the surface-sterilization technique most efficient for eliminating epiphytes, to document endophytes of healthy tissues from Glaycyrrhiza inflat Bat. in Xinjiang. Surface sterilization with 5% commercial solution of sodium hypochlorite for 5 minutes was reaffirmed as adequate for removing epiphytes on licorice roots. From the 151 segments incubated, 149 bacterial isolates and 2 fungal isolates were obtained. From all the isolates, Bacterial isolates were identified by VITEK-AMS. Part of Bacteria were identified in 13 different genus. Fungal species were characterized as Penicillium sp. and Fusarium sp.with microscope. |
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| Keywords: | Endophytes Glaycyrrhiza inflat Bat Symptomless licorice tissues |
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