Interferon inhibits the accumulation of glycerol phosphate dehydrogenase mRNA in oligodendrocytes and C6 cells |
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| Authors: | A. C. Passaquin G. Coupin W. A. Schreier P. Poindron R. A. Cole J. de Vellis |
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| Affiliation: | (1) Departments of Anatomy and Cell Biology, and Psychiatry, UCLA School of Medicine. Laboratory of Biomedical and Environmental Sciences, Mental Retardation Research Center, and Brain Research Institute, University of California, 760 Westwood Plaza, 90024-1759 Los Angeles, CA, USA;(2) Faculté de Pharmacie, Laboratoire de Virologie, 67400 Illkirch-Graffenstaden, France |
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| Abstract: | We investigated the effect of rat interferon- / (IFN) on the expression of glycerol phosphate dehydrogenase (E.C.1.1.1.8; GPDH), in both C6 cells and pure cultures of oligodendrocytes. IFNs are naturally produced inhibitors of cell growth that can also affect differentiated cell functions. GPDH is a biochemical marker for oligodendrocytes and is known to be developmentally regulated and steroid inducible. GPDH activity is induced by hydrocortisone (HC) 3.5 fold in C6 cells and 5 fold in oligodendrocytes compared to untreated cultures. A pretreatment of these cells with 75 U/ml of rat IFN-/ resulted in an inhibition of the HC induction of GPDH enzymatic activity by 50% and 40% in C6 cells and oligodendrocytes respectively. We also found that IFN impaired the accumulation of GPDH mRNA in both cell types. These results demonstrate that IFNs are capable of modifying the cellular response to hormones in cells of neuroepithelial origin, and suggest the possibility that IFNs may be able to influence the development and function of the brain.Special issue dedicated to Dr. Paola S. Timiras |
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| Keywords: | Interferon glycerol phosphate dehydrogenase hydrocortisone C6 cells oligodendrocytes |
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