Expression of Cholera Toxin B Subunit in Transgenic Rice Endosperm |
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| Authors: | Maria Oszvald Tae-Jin Kang Sandor Tomoskozi Barnabas Jenes Tae-Geum Kim Youn-Soo Cha Laszlo Tamas Moon-Sik Yang |
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| Institution: | (1) Department of Biochemistry and Food Technology, Budapest University of Technology and Economics, Budapest, Hungary;(2) Department of Plant Physiology and Molecular Plant Biology, Eotvos Lorand University, Budapest, Hungary;(3) Department of Food and Nutrition, Chonbuk National University, Jeonju, 561-756, South Korea;(4) Agricultural Biotechnology Center, Godollo, Hungary;(5) Division of Biological Science and the Research Center for Bioactive Materials, Chonbuk National University, 664-14, Deokjin-Dong, Deokjin-Ku, Jeonju, 561-756, South Korea; |
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| Abstract: | The synthetic cholera toxin B subunit (CTB) gene, modified according to the optimized codon usage of plant genes, was introduced
into a plant expression vector and expressed under the control of the Bx17 HMW (high molecular weight) wheat endosperm-specific
promoter containing an intron of the rice act1. The recombinant vector was transformed into rice plants using a biolistic-mediated transformation method. Stable integration
of the synthetic CTB gene into the chromosomal DNA was confirmed by PCR amplification analysis. A high level of CTB (2.1%
of total soluble protein) was expressed in the endosperm tissue of the transgenic rice plants. The synthetic CTB produced
only in the rice endosperm demonstrated strong affinity for GM1-ganglioside, thereby suggesting that the CTB subunits formed an active pentamer. The successful expression of CTB genes in
transgenic plants makes it a powerful tool for the development of a plant-derived edible vaccine. |
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| Keywords: | Cholera toxin B subunit Rice Endosperm-specific promoter Plant-based vaccine |
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